mouse tnf α paired antibodies  (R&D Systems)

Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

doi: 10.1016/j.ijpddr.2026.100642

Figure Lengend Snippet: Scatter plots of the association between TNF-α production and the IC 50 values (LDA and LMA) Scatter plots showing the association between TNF-α production percentage (expressed relative to the control) and the IC 50 values obtained in (left) the Larval Development Assay (LDA) and (right) the Larval Migration Assay (LMA) for six terpene compounds (anethole, cinnamaldehyde, menthol, carvacrol, eugenol and thymol). Each point represents the mean IC 50 and TNFα production for a given compound, and horizontal/vertical bars indicate the corresponding confidence intervals. Lower IC 50 values reflect higher antiparasitic potency.

Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

Techniques: Control, Migration

Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: Terpenic compounds possess anthelmintic and immunomodulatory properties with potential for controlling equine cyathostomin infections

doi: 10.1016/j.ijpddr.2026.100642

Figure Lengend Snippet: Anti-inflammatory activity of carvacrol and cinnamaldehyde on equine PBMC Boxplots showing TNF-α concentrations (ng/mL) measured in equine peripheral blood mononuclear cells (PBMCs) exposed to DMSO (0.05%), LPS (125 ng/mL), the combination of DMSO and LPS, carvacrol (5 μg/mL), cinnamaldehyde (5 μg/mL), the combination of either compound with LPS, and the untreated condition (control). Points represent individual replicates from four independent assays. Asterisks indicate significant differences relative to the corresponding control condition (∗ P = 0.01, ∗∗ P < 0.001).

Article Snippet: The cells were then incubated at +37 °C (5% CO 2 ) for 24 h. After incubation, the concentration of TNF-α in the medium for each condition was quantified by ELISA using mouse TNF-α paired antibodies (R and D Systems DY410).

Techniques: Activity Assay, Control

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Exosomal PDL1 Suppresses the Anticancer Activity of CD8 + T Cells in Hepatocellular Carcinoma

doi: 10.1155/2024/1608582

Figure Lengend Snippet: Regulatory effect of exosomal PDL1 on proliferation of CD8 + T and hepatic cancer cells. (A–D): qRT-PCR (A and C) and western blot (B and D) for PDL1 level in Huh7 cells with the overexpression or knockdown of PDL1. (E) Western blot for exosomal PDL1 level in supernatants of PDL1-silenced Huh7 cells. (F and G): TEM (F) and NTA (G) for isolated exosomes derived from the supernatants of PDL1-silenced Huh7 cells. (H) Proliferation of Huh7 cells with the overexpression or silencing of PDL1, as assayed by CCK8. (I) Proliferation of CD8 + T cells with the treatments of exosomes derived from the supernatants of Huh7 cells, as assayed by CCK8. PDL1 was overexpressed or silenced in Huh7 cells. (J) ELISA for TNF α and IFN- γ levels in supernatants of human CD8 + T cells after treatments with exosomes. Data were analyzed by Student's two-sided t -test and are represented as mean ± SD. ∗∗ p <0.01, ∗∗∗ p <0.001.

Article Snippet: ELISA plates (96-well) (Biolegend) that coated with Human PDL1 antibody (DY156, R&D SYSTEMS, Minneapolis, MN, USA), Human TNF α ELISA Kit (KIT10602, SinoBiology, Beijing, China), Mouse TNF α Matched ELISA Antibody Pair Set (SEKA50349, SinoBiology), Human IFN gamma ELISA Kit (KIT11725A, SinoBiology), and Mouse IFN gamma ELISA Kit (KIT50709, SinoBiology) were used for detecting protein levels of PDL1, TFN α , and IFN- γ in samples according to manufactures' instructions.

Techniques: Quantitative RT-PCR, Western Blot, Over Expression, Knockdown, Isolation, Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Exosomal PDL1 Suppresses the Anticancer Activity of CD8 + T Cells in Hepatocellular Carcinoma

doi: 10.1155/2024/1608582

Figure Lengend Snippet: Isolation of exosomes derived from the supernatants of cultured Hepa1–6 cells with different levels of PDL1. (A–D): qRT-PCR (A and C) and western blot (B and D) for PDL1 level in Hepa1–6 cells with the overexpression or knockdown of PDL1. (E) Western blot for protein level of PDL1, TSG101, and GAPDH in exosomes derived from the supernatants of PDL1-silenced Hepa1–6 cells. (F and G) TEM and NTA analysis for the isolated exosomes derived from the supernatants of Pdl1-silenced Hepa1–6 cells. (H) Proliferation of Hepa1–6 cells with the overexpression or silencing of Pdl1, as assayed by CCK8. (I) Proliferation of CD8 + T cells after treatments of exosomes derived from the supernatants of Hepa1–6 cells, as assayed by CCK8. Pdl1 was overexpressed or silenced in Hepa1–6 cells. (J) ELISA for TNF α and IFN- γ levels in supernatants of mice CD8 + T cells after treatments with exosomes. Data were analyzed by Student's two-sided t -test and are represented as mean ± SD. ∗∗∗ p <0.001.

Article Snippet: ELISA plates (96-well) (Biolegend) that coated with Human PDL1 antibody (DY156, R&D SYSTEMS, Minneapolis, MN, USA), Human TNF α ELISA Kit (KIT10602, SinoBiology, Beijing, China), Mouse TNF α Matched ELISA Antibody Pair Set (SEKA50349, SinoBiology), Human IFN gamma ELISA Kit (KIT11725A, SinoBiology), and Mouse IFN gamma ELISA Kit (KIT50709, SinoBiology) were used for detecting protein levels of PDL1, TFN α , and IFN- γ in samples according to manufactures' instructions.

Techniques: Isolation, Derivative Assay, Cell Culture, Quantitative RT-PCR, Western Blot, Over Expression, Knockdown, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Helicobacter pylori CagA promotes immune evasion of gastric cancer by upregulating PD-L1 level in exosomes

doi: 10.1016/j.isci.2023.108414

Figure Lengend Snippet:

Article Snippet: TNF-alpha Matched ELISA Antibody Pair Set, Mouse , Sino Biological , Cat# SEK50349.

Techniques: Purification, Virus, Recombinant, Western Blot, Marker, Cell Isolation, Enzyme-linked Immunosorbent Assay, Preserving, shRNA, Sequencing, Software